Anticorps Monoclonal anti-ATL3

• Phare
• Validé par KD/KO

ATL3 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E7C4

N° de cat : 68516-1-PBS

Synonymes

EC:3.6.5.-, Atlastin-3, Atlastin 3, 2E7C4

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Galerie de données de validation

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  WB analysis using 68516-1-Ig (same clone as 68516-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68516-1-Ig (ATL3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68516-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 68516-1-Ig (same clone as 68516-1-PBS)

  WB result of ATL3 antibody (68516-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATL3 transfected Jurkat cells. This data was developed using the same antibody clone with 68516-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68516-1-Ig (same clone as 68516-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (68516-1-Ig, Clone: 2E7C4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68516-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50703-1

  Cytometric bead array standard curve of MP50703-1, ATL3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68516-2-PBS. Detection antibody: 68516-1-PBS. Standard:Ag10248. Range: 0.781-100 ng/mL.

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Informations sur le produit

68516-1-PBS cible ATL3 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATL3 Protéine recombinante Ag10248
• Nom complet: atlastin GTPase 3
• Masse moléculaire calculée: 541 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC077727
• Symbole du gène: ATL3
• Identification du gène (NCBI): 25923
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.

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