Anticorps Polyclonal de lapin anti-ATM

• Phare
• Validé par KD/KO

ATM Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(43)

N° de cat : 27156-1-AP

Synonymes

Serine-protein kinase ATM, EC:2.7.11.1, A-T mutated, A T mutated

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Galerie de données de validation

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  WB analysis using 27156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27156-1-AP (ATM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27156-1-AP

  WB result of ATM antibody (27156-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and sg-ATM transfected A549 cells.

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  WB analysis of HEK-293 using 27156-1-AP

  WB result of ATM antibody (27156-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATM transfected HEK-293 cells.

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  IP experiment of HEK-293 using 27156-1-AP

  IP result of anti-ATM (IP:27156-1-AP, 4ug; Detection:27156-1-AP 1:800) with HEK-293 cells lysate 1480 ug.

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  IHC staining of human breast cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27156-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27156-1-AP (ATM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 41 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

27156-1-AP cible ATM dans les applications de WB, IHC, IF, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATM Protéine recombinante Ag25909
• Nom complet: ataxia telangiectasia mutated
• Poids moléculaire observé: 310-350 kDa
• Numéro d’acquisition GenBank: BC137169
• Symbole du gène: ATM
• Identification du gène (NCBI): 472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.

Publications

Species	Application	Title
human	WB	Sci Transl Med; Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer; Authors - Jia-Han Ding
mouse	WB	Acta Pharm Sin B; Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis; Authors - Yujun Zhou
human	WB	Cell Death Differ; p53-dependent elimination of aneuploid mitotic offspring by entosis.; Authors - Jianqing Liang
human	WB	Oncogene; TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex; Authors - Ya Wang
	WB	Clin Transl Med; Single-cell profiling-guided combination therapy of c-Fos and histone deacetylase inhibitors in diffuse large B-cell lymphoma.; Authors - Luqiao Wang
		EBioMedicine; High PARP-1 expression predicts poor survival in acute myeloid leukemia and PARP-1 inhibitor and SAHA-bendamustine hybrid inhibitor combination treatment synergistically enhances anti-tumor effects.; Authors - Xia Li

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