Anticorps Monoclonal anti-ATM

ATM Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

3E6F4

N° de cat : 67586-1-Ig

Synonymes

3E6F4, A T mutated, A-T mutated, EC:2.7.11.1, Serine-protein kinase ATM

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Galerie de données de validation

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  WB analysis using 67586-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67586-1-Ig (ATM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67586-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67586-1-Ig (ATM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

67586-1-Ig cible ATM dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATM Protéine recombinante Ag30001
• Nom complet: ataxia telangiectasia mutated
• Poids moléculaire observé: 310-350 kDa
• Numéro d’acquisition GenBank: BC137169
• Symbole du gène: ATM
• Identification du gène (NCBI): 472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.

Publications

Species	Application	Title
human	WB	Food Chem Toxicol; DON entry into the nucleus induces DNA damage, apoptosis and cycle arrest in GES-1 cells; Authors - Silu Hou
rat	WB,IF	CNS Neurosci Ther; Multiple sevoflurane exposures during mid-trimester induce neurotoxicity in the developing brain initiated by 15LO2-Mediated ferroptosis; Authors - Qian Jiang
human	WB	J Mol Med (Berl); High expression of BCAT1 sensitizes AML cells to PARP inhibitor by suppressing DNA damage response; Authors - Jiajia Pan
pig	WB	Signal Transduct Target Ther; In vivo evaluation of guide-free Cas9-induced safety risks in a pig model; Authors - Weikai Ge

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