Anticorps Recombinant de lapin anti-ATM

ATM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240560H8

N° de cat : 83608-4-PBS

Synonymes

240560H8, A T mutated, A-T mutated, EC:2.7.11.1, Serine-protein kinase ATM

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00592-4

  Sandwich ELISA standard curve of MP00592-4, Human ATM Recombinant Matched Antibody Pair - PBS only. 83608-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25920. 83608-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP00592-4

  The mean ATM concentration was determined to be 3,383.80 pg/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load and 3,536.69 pg/mL in MDA-MB-468 cell extract based on a 3.20 mg/mL extract load.

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  IF Staining of HeLa using 83608-4-RR (same clone as 83608-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATM antibody (83608-4-RR, Clone: 240560H8 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83608-4-RR (same clone as 83608-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATM Recombinant Antibody (83608-4-RR, Clone:240560H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83608-4-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83608-4-RR (same clone as 83608-4-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ATM Recombinant Antibody (83608-4-RR, Clone:240560H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83608-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83608-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Human ATM were performed. The affinity constant is 2.58 nM.

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Informations sur le produit

83608-4-PBS cible ATM dans les applications de IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATM Protéine recombinante Ag25920
• Nom complet: ataxia telangiectasia mutated
• Numéro d’acquisition GenBank: BC137169
• Symbole du gène: ATM
• Identification du gène (NCBI): 472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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