Anticorps Recombinant de lapin anti-ATM

• Phare
• Validé par KD/KO

ATM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240560F5

N° de cat : 83608-5-PBS

Synonymes

240560F5, A T mutated, A-T mutated, EC:2.7.11.1, Serine-protein kinase ATM

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Galerie de données de validation

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  WB analysis using 83608-5-RR (same clone as 83608-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83608-5-RR (ATM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 83608-5-RR (same clone as 83608-5-PBS)

  WB result of ATM antibody (83608-5-RR; 1:3000; room temperature for 1.5 hours) with wild-type and sg-ATM transfected A549 cells. This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83608-5-RR (same clone as 83608-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83608-5-RR (ATM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 83608-5-RR (same clone as 83608-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83608-5-RR (same clone as 83608-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83608-5-RR (same clone as 83608-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83608-5-RR (same clone as 83608-5-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ATM Recombinant antibody (83608-5-RR, Clone:240560F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83608-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83608-5-RR against Human ATM were performed. The affinity constant is 0.318 nM.

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Informations sur le produit

83608-5-PBS cible ATM dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATM Protéine recombinante Ag25920
• Nom complet: ataxia telangiectasia mutated
• Poids moléculaire observé: 310-350 kDa
• Numéro d’acquisition GenBank: BC137169
• Symbole du gène: ATM
• Identification du gène (NCBI): 472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.

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