Anticorps Polyclonal de lapin anti-ATOX1

ATOX1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22641-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 22641-1-AP (same clone as 22641-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22641-1-AP (ATOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22641-1-PBS in a different storage buffer formulation.

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  WB analysis using 22641-1-AP (same clone as 22641-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22641-1-AP (ATOX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22641-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 22641-1-AP (same clone as 22641-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 22641-1-AP (ATOX1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22641-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 22641-1-AP (same clone as 22641-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 22641-1-AP (ATOX1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22641-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 22641-1-AP (same clone as 22641-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22641-1-AP (ATOX1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22641-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 22641-1-AP (same clone as 22641-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ATOX1 antibody (22641-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 22641-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22641-1-PBS cible ATOX1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATOX1 Protéine recombinante Ag18460
• Nom complet: ATX1 antioxidant protein 1 homolog (yeast)
• Masse moléculaire calculée: 68 aa, 8 kDa
• Poids moléculaire observé: 7 kDa
• Numéro d’acquisition GenBank: BC112248
• Symbole du gène: ATOX1
• Identification du gène (NCBI): 475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Antioxidant 1 (ATOX1) is a copper chaperone to regulate copper homeostasis in cell. In the cytosol, ATOX1 always binds to copper and transfer to ATPase proteins in the trans-Golgi network, thereby promoting copper supply to various copper-dependent oxidereductases matured within the secretory vesicles (PMID: 28294521). Also, the protein could protect cells against oxidative damage and the expression levels of ATOX1 may help to the inflammatory response and antioxidant defense,even to modulate response to the cancer (PMID: 27472369). ATOX1 plays an important role in the physiology of the human central nervous system ,and it's highly expressed in the cerebral cortex and hippocampus (PMID: 30545441). Either cytosol or nucleus location of ATOX1 has been reported in different literations, which may be associated with cell status (PMID: 24445997; 31317143).

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