Anticorps Recombinant de lapin anti-ATOX1

ATOX1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240679E9

N° de cat : 83785-6-RR

Synonymes

ATX1, ATOX 1, Antioxidant 1, 240679E9

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Galerie de données de validation

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  WB analysis using 83785-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83785-6-RR (ATOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of PC-3 using 83785-6-RR

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ATOX1 Recombinant antibody (83785-6-RR, Clone:240679E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HeLa using 83785-6-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATOX1 Recombinant antibody (83785-6-RR, Clone:240679E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83785-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83785-6-RR against Human ATOX1 were performed. The affinity constant is 183 nM.

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Applications testées

• Résultats positifs en WB: cellules A375, cellules A549, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules NCI-H1299
• Résultats positifs en FC (Intra): cellules HeLa, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83785-6-RR cible ATOX1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATOX1 Protéine recombinante Ag18460
• Nom complet: ATX1 antioxidant protein 1 homolog (yeast)
• Masse moléculaire calculée: 68 aa, 8 kDa
• Poids moléculaire observé: 7 kDa
• Numéro d’acquisition GenBank: BC112248
• Symbole du gène: ATOX1
• Identification du gène (NCBI): 475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Antioxidant 1 (ATOX1) is a copper chaperone to regulate copper homeostasis in cell. In the cytosol, ATOX1 always binds to copper and transfer to ATPase proteins in the trans-Golgi network, thereby promoting copper supply to various copper-dependent oxidereductases matured within the secretory vesicles (PMID: 28294521). Also, the protein could protect cells against oxidative damage and the expression levels of ATOX1 may help to the inflammatory response and antioxidant defense,even to modulate response to the cancer (PMID: 27472369). ATOX1 plays an important role in the physiology of the human central nervous system ,and it's highly expressed in the cerebral cortex and hippocampus (PMID: 30545441). Either cytosol or nucleus location of ATOX1 has been reported in different literations, which may be associated with cell status (PMID: 24445997; 31317143).

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