Anticorps Monoclonal anti-ATP1A1/2

ATP1A1/2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3D8G1

N° de cat : 68505-1-PBS

Synonymes

ATP1A2, 3D8G1, alpha 2 ATPase, ATP1A 2, EC:7.2.2.13

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Galerie de données de validation

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  WB analysis using 68505-1-Ig (same clone as 68505-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  WB analysis of SK-BR-3 using 68505-1-Ig (same clone as 68505-1-PBS)

  SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 68505-1-Ig (same clone as 68505-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 68505-1-Ig (same clone as 68505-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68505-1-Ig (ATP1A1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 68505-1-Ig (same clone as 68505-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using ATP1A1/2 antibody (68505-1-Ig, Clone: 3D8G1 ) at dilution of 1:450 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68505-1-Ig (same clone as 68505-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP1A1/2 antibody (68505-1-Ig, Clone: 3D8G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68505-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68505-1-PBS cible ATP1A1/2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATP1A1/2 Protéine recombinante Ag10489
• Nom complet: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• Masse moléculaire calculée: 1020 aa, 112 kDa
• Poids moléculaire observé: 97-100 kDa
• Numéro d’acquisition GenBank: BC052271
• Symbole du gène: ATP1A2
• Identification du gène (NCBI): 477
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP1A1/2 is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody can recognize ATP1A1 and ATP1A2.

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