Anticorps Monoclonal anti-ATP1A1

ATP1A1 Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E9B7

N° de cat : 68735-2-PBS

Synonymes

1E9B7, ATP1A 1, Atpa 1, EC:7.2.2.13, Na(+)/K(+) ATPase 1

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Galerie de données de validation

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  WB analysis using 68735-2-Ig (same clone as 68735-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68735-2-Ig (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68735-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50088-1

  Sandwich ELISA standard curve of MP50088-1, ATP1A1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68735-1-PBS. Detection antibody: HRP-conjugated 68735-2-PBS. Standard:Ag5990. Range: 0.195-50 ng/mL.

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  IHC staining of mouse kidney using 68735-2-Ig (same clone as 68735-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68735-2-Ig (ATP1A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68735-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68735-2-Ig (same clone as 68735-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68735-2-Ig (ATP1A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68735-2-PBS in a different storage buffer formulation.

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Informations sur le produit

68735-2-PBS cible ATP1A1 dans les applications de WB, IHC, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATP1A1 Protéine recombinante Ag5990
• Nom complet: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• Masse moléculaire calculée: 113 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC050359
• Symbole du gène: ATP1A1
• Identification du gène (NCBI): 476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

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