Anticorps Recombinant de lapin anti-ATP1A1

ATP1A1 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240160B5

N° de cat : 83191-5-RR

Synonymes

240160B5, ATP1A 1, Atpa 1, EC:7.2.2.13, Na(+)/K(+) ATPase 1

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Galerie de données de validation

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  IF Staining of A431 using 83191-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-5-RR, Clone: 240160B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A431 using 83191-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-5-RR, Clone: 240160B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HEK-293 using 83191-5-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug ATP1A1 Recombinant antibody (83191-5-RR, Clone:240160B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83191-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83191-5-RR against Human ATP1A1 were performed. The affinity constant is 87.7 pM.

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Applications testées

• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83191-5-RR cible ATP1A1 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATP1A1 Protéine recombinante Ag5763
• Nom complet: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• Masse moléculaire calculée: 113 kDa
• Numéro d’acquisition GenBank: BC050359
• Symbole du gène: ATP1A1
• Identification du gène (NCBI): 476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein.

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