Anticorps Polyclonal de lapin anti-ATP1A2

ATP1A2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 16836-1-AP

Synonymes

alpha 2 ATPase, ATP1A2, FHM2, KIAA0778, MHP2, Sodium pump subunit alpha 2

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Galerie de données de validation

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  WB analysis using 16836-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16836-1-AP (ATP1A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16836-1-AP

  37 ℃ incubated or boiled mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 16836-1-AP (ATP1A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 16836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16836-1-AP (ATP1A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16836-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16836-1-AP (ATP1A2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 16836-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16836-1-AP (ATP1A2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 16836-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16836-1-AP (ATP1A2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16836-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16836-1-AP (ATP1A2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16836-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16836-1-AP (ATP1A2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 16836-1-AP

  Immunofluorescent analysis of Hela cells, using ATP1A2 antibody 16836-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 16836-1-AP

  1X10^6 HeLa cells were stained with 0.5ug ATP1A2 antibody (16836-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris incubé à 37 °C, tissu de muscle squelettique de souris incubé à 37 °C
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu cutané humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.05 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

16836-1-AP cible ATP1A2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP1A2 Protéine recombinante Ag10515
• Nom complet: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• Masse moléculaire calculée: 1020 aa, 112 kDa
• Poids moléculaire observé: 97-100 kDa
• Numéro d’acquisition GenBank: BC052271
• Symbole du gène: ATP1A2
• Identification du gène (NCBI): 477
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP1A2 (Na+/K+-ATPase α-2 subunit) is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody was raised against the internal region of the human ATP1A2 and can recognizes all the isoforms of α subunit. The 65kDa band detected occasionally may be the degradation product of ATP1A2.

Publications

Species	Application	Title
human	WB	Nat Commun; Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.; Authors - Yi-Ing Chen
human	IHC	Eur J Nucl Med Mol Imaging; Tumour blood flow for prediction of human prostate cancer aggressiveness: a study with Rubidium-82 PET, MRI and Na+/K+-ATPase-density.; Authors - Mads Ryø Jochumsen
canine	WB	J Cereb Blood Flow Metab; Evaluating the involvement of cerebral microvascular endothelial Na+/K+-ATPase and Na+-K+-2Cl- co-transporter in electrolyte fluxes in an in vitro blood-brain barrier model of dehydration.; Authors - Kasper Lykke
mouse	IHC,IF	Glia; The α2β2 isoform combination dominates the astrocytic Na(+) /K(+) -ATPase activity and is rendered nonfunctional by the α2.G301R familial hemiplegic migraine type 2-associated mutation.; Authors - Anca Stoica
human	WB,IF	Biomed Pharmacother; Rebound effects of NCX3 pharmacological inhibition: A novel strategy to accelerate myelin formation in oligodendrocytes.; Authors - Mariarosaria Cammarota
human,mouse	WB,IHC,IF	J Cell Mol Med; Comprehensive analysis identified a reduction in ATP1A2 mediated by ARID3A in abdominal aortic aneurysm.; Authors - Qunhui Wang

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