Anticorps Recombinant de lapin anti-ATP1A2

ATP1A2 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242414G12

N° de cat : 84999-4-PBS

Synonymes

242414G12, alpha 2 ATPase, ATP1A 2, EC:7.2.2.13, Na(+)/K(+) ATPase alpha-2 subunit

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Galerie de données de validation

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  WB analysis using 84999-4-RR (same clone as 84999-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84999-4-RR (ATP1A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84999-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84999-4-RR (same clone as 84999-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84999-4-RR (ATP1A2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84999-4-PBS in a different storage buffer formulation.

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Informations sur le produit

84999-4-PBS cible ATP1A2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATP1A2 Protéine recombinante Ag10489
• Nom complet: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• Masse moléculaire calculée: 1020 aa, 112 kDa
• Poids moléculaire observé: 97-100 kDa
• Numéro d’acquisition GenBank: BC052271
• Symbole du gène: ATP1A2
• Identification du gène (NCBI): 477
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP1A2 (Na+/K+-ATPase α-2 subunit) is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4. The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. For optimal WB detection with ATP1A2, we do not recommend boiling the sample after lysis.

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