Anticorps Polyclonal de lapin anti-ATP1A2-Specific

• Phare
• Validé par KD/KO

ATP1A2-Specific Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55179-1-PBS

Synonymes

ATP1A2, Sodium pump subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2

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Galerie de données de validation

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  WB analysis of mouse brain using 55179-1-AP (same clone as 55179-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 55179-1-AP (same clone as 55179-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 55179-1-AP (same clone as 55179-1-PBS)

  IP result of anti-ATP1A2-Specific (IP:55179-1-AP, 4ug; Detection:55179-1-AP 1:800) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 55179-1-AP (same clone as 55179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55179-1-AP (ATP1A2-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55179-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55179-1-PBS cible ATP1A2-Specific dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• Masse moléculaire calculée: 112 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: NM_000702
• Symbole du gène: ATP1A2
• Identification du gène (NCBI): 477
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP1A2, also named as KIAA0778, belongs to the cation transport ATPase (P-type) family and Type IIC subfamily. It is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. Defects in ATP1A2 are the cause of familial hemiplegic migraine type 2 (FHM2). Defects in ATP1A2 are a cause of alternating hemiplegia of childhood (AHC). This antibody is specific to ATP1A2.

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