Anticorps Polyclonal de lapin anti-ATP1A3 (C-terminal)

ATP1A3 (C-terminal) Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10868-1-PBS

Synonymes

ATP1A3, ATP1A3 (middle), Alpha(III), ATP1A 3, DYT12

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Galerie de données de validation

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  WB analysis of C2C12 using 10868-1-AP (same clone as 10868-1-PBS)

  C2C12 cell were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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  WB analysis using 10868-1-AP (same clone as 10868-1-PBS)

  Mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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  WB analysis using 10868-1-AP (same clone as 10868-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10868-1-AP (same clone as 10868-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10868-1-AP (same clone as 10868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10868-1-AP (same clone as 10868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10868-1-AP (ATP1A3 (C-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10868-1-AP (same clone as 10868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10868-1-AP (same clone as 10868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 10868-1-AP (same clone as 10868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 10868-1-AP (same clone as 10868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 10868-1-AP (same clone as 10868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 10868-1-AP (same clone as 10868-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 10868-1-AP (ATP1A3 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 10868-1-AP (same clone as 10868-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10868-1-AP (ATP1A3 (C-terminal) antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 10868-1-AP (same clone as 10868-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10868-1-AP (ATP1A3 (C-terminal) antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10868-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10868-1-PBS cible ATP1A3 (C-terminal) dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP1A3 (C-terminal) Protéine recombinante Ag1313
• Nom complet: ATPase, Na+/K+ transporting, alpha 3 polypeptide
• Masse moléculaire calculée: 113 kDa
• Poids moléculaire observé: 100-113 kDa
• Numéro d’acquisition GenBank: BC015566
• Symbole du gène: ATP1A3
• Identification du gène (NCBI): 478
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP1A3 participates in the catalyticing hydrolysis of ATP and the exchanging of sodium and potassium ions across plasma membrane. The catalyticing activity mode is ATP + H2O + Na⁺(In) + K⁺(Out) = ADP + phosphate + Na⁺(Out) + K⁺(In). It has been published that the neurologic disorders rapid-onset dystonia-parkionsonism (RDP), alternating hemiplegia of childhood (ACH) and CAPOS syndrome (cerebellar ataxia, areflexia, pes cavus, optic atrophy and sensorineural hearing loss) are all related with the mutation of ATP1A3. There are other reports suggest that early life epilepsy and episodic apnea revealing are potentially associated with the mutation of ATP1A3 as a result of impairment of Na⁺/K⁺ homeostasis. This antibody is generated against the C-terminal region (665-1013aa) of ATP1A3 and detects the band around 100-113 kDa in SDS-PAGE.(PMID: 30097153, 20301294, 29922587)

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