Anticorps Recombinant de lapin anti-ATP1B1

ATP1B1 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242109D12

N° de cat : 84707-5-PBS

Synonymes

242109D12, ATP1B 1, Beta 1 subunit of Na(+) K(+) ATPase, Na, K-ATPase β1, Na+/K+ ATPase beta 1 subunit

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Galerie de données de validation

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  WB analysis using 84707-5-RR (same clone as 84707-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84707-5-RR (ATP1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84707-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84707-5-RR (same clone as 84707-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84707-5-RR (ATP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84707-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84707-5-RR (same clone as 84707-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84707-5-RR (ATP1B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84707-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84707-5-RR (same clone as 84707-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84707-5-RR (ATP1B1 antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 84707-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 84707-5-RR (same clone as 84707-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84707-5-RR (ATP1B1 antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 84707-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84707-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84707-5-RR against Human ATP1B1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84707-5-PBS cible ATP1B1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATP1B1 Protéine recombinante Ag7279
• Nom complet: ATPase, Na+/K+ transporting, beta 1 polypeptide
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 45-52 kDa
• Numéro d’acquisition GenBank: BC000006
• Symbole du gène: ATP1B1
• Identification du gène (NCBI): 481
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)

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