Anticorps Polyclonal de lapin anti-ATP1B2

ATP1B2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 22338-1-AP

Synonymes

Adhesion molecule in glia, ATPase Na+/K+ beta 2, Sodium/potassium-dependent ATPase subunit beta-2, Sodium/potassium-transporting ATPase subunit beta-2

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Galerie de données de validation

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  WB analysis of mouse brain using 22338-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22338-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 22338-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 22338-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 22338-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 22338-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 22338-1-AP

  IP result of anti-ATP1B2 (IP:22338-1-AP, 4ug; Detection:22338-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human brain using 22338-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 22338-1-AP (ATP1B2 Antibody) at dilution of 1:50.

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  IHC staining of human brain using 22338-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 22338-1-AP (ATP1B2 Antibody) at dilution of 1:50.

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  IF Staining of mouse brain using 22338-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ATP1B2 antibody (22338-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules C2C12, cellules C6, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IF: See 2 publications below

Informations sur le produit

22338-1-AP cible ATP1B2 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP1B2 Protéine recombinante Ag17818
• Nom complet: ATPase, Na+/K+ transporting, beta 2 polypeptide
• Masse moléculaire calculée: 290 aa, 33 kDa
• Poids moléculaire observé: 45-65 kDa
• Numéro d’acquisition GenBank: BC126175
• Symbole du gène: ATP1B2
• Identification du gène (NCBI): 482
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP1B2 is the β2 subunit of Na+/K+-ATPase which is an essential membrane-bound enzyme responsible for the transport of Na+ and K+ in most eukaryotic cells. ATP1B2 is also called the adhesion molecule on glia (AMOG) and it is highly expressed in normal glia. It is a heavily glycosylated protein that plays a role in cellular adhesion in the CNS. Recently differential expression of ATP1B2 has been found in some glioneuronal tumors (PMID: 23887941, 19371356). This antibody recognizes the endogenous ATP1B2 protein in human brain. The bands between 45 kDa and 65 kDa represent the glycosylated forms of ATP1B2 in different levels (PMID: 8918259).

Publications

Species	Application	Title
mouse	IF	Cell Mol Gastroenterol Hepatol; Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells.; Authors - Yuli Wang
rat	WB	Redox Biol; Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liver sinusoidal endothelial cells defenestration.; Authors - Xiaoying Luo
human	WB	Cancer Cell Int; Spider venom components decrease glioblastoma cell migration and invasion through RhoA-ROCK and Na+/K+-ATPase β2: potential molecular entities to treat invasive brain cancer.; Authors - Natália Barreto
human,rat	WB	World J Biol Psychiatry; Altered expression levels of miR-144-3p and ATP1B2 are associated with schizophrenia.; Authors - Bo Pan
human	WB	Eur J Appl Physiol; Resistance training upregulates skeletal muscle Na+, K+-ATPase content, with elevations in both α1 and α2, but not β isoforms.; Authors - Muath M Altarawneh
rat	WB	Neurosci Lett; Effects of icariin on alleviating schizophrenia-like symptoms by regulating the miR-144-3p/ATP1B2/mTOR signalling pathway; Authors - Bo Pan

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