Anticorps Monoclonal anti-ATP1B3

• Phare
• Validé par KD/KO

ATP1B3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1H9C1

N° de cat : 67554-1-Ig

Synonymes

1H9C1, ATPB 3, ATPB-3, CD298, Sodium/potassium-dependent ATPase subunit beta-3

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Galerie de données de validation

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  WB analysis of Jurkat using 67554-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 67554-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67554-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67554-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67554-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 67554-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 67554-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67554-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67554-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 67554-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1B3 antibody (67554-1-Ig, Clone: 1H9C1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules HepG2, cellules THP-1, tissu placentaire humain
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

67554-1-Ig cible ATP1B3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATP1B3 Protéine recombinante Ag30127
• Nom complet: ATPase, Na+/K+ transporting, beta 3 polypeptide
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32-43 kDa
• Numéro d’acquisition GenBank: BC011835
• Symbole du gène: ATP1B3
• Identification du gène (NCBI): 483
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).

Publications

Species	Application	Title
human	IHC	Front Immunol; Integrative Transcriptomic, Proteomic and Functional Analysis Reveals ATP1B3 as a Diagnostic and Potential Therapeutic Target in Hepatocellular Carcinoma.; Authors - Shanshan Lu ; KD Validated

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