Anticorps Monoclonal anti-ATP1B3

• Phare
• Validé par KD/KO

ATP1B3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H9C1

N° de cat : 67554-1-PBS

Synonymes

1H9C1, ATPB 3, ATPB-3, CD298, Sodium/potassium-dependent ATPase subunit beta-3

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Galerie de données de validation

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  WB analysis of Jurkat using 67554-1-Ig (same clone as 67554-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 67554-1-Ig (same clone as 67554-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67554-1-Ig (same clone as 67554-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67554-1-Ig (same clone as 67554-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67554-1-Ig (same clone as 67554-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67554-1-Ig (ATP1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67554-1-Ig (ATP1B3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67554-1-Ig (same clone as 67554-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1B3 antibody (67554-1-Ig, Clone: 1H9C1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67554-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67554-1-PBS cible ATP1B3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATP1B3 Protéine recombinante Ag30127
• Nom complet: ATPase, Na+/K+ transporting, beta 3 polypeptide
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32-43 kDa
• Numéro d’acquisition GenBank: BC011835
• Symbole du gène: ATP1B3
• Identification du gène (NCBI): 483
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).

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