Anticorps Recombinant de lapin anti-ATP2A1

ATP2A1 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241500D12

N° de cat : 84213-5-PBS

Synonymes

241500D12, ATP2A, ATP2A 1, Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, EC:7.2.2.10

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Galerie de données de validation

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  WB analysis using 84213-5-RR (same clone as 84213-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84213-5-RR (ATP2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84213-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84213-5-RR (same clone as 84213-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84213-5-RR (ATP2A1 antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84213-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 84213-5-RR (same clone as 84213-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using ATP2A1 antibody (84213-5-RR, Clone: 241500D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84213-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84213-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84213-5-RR against Human ATP2A1 were performed. The affinity constant is 6.90 nM.

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Informations sur le produit

84213-5-PBS cible ATP2A1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATP2A1 Protéine recombinante Ag17944
• Nom complet: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
• Masse moléculaire calculée: 1001 aa, 110 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC037354
• Symbole du gène: ATP2A1
• Identification du gène (NCBI): 487
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP2A1 also known as SERCA1, encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen and is involved in muscular excitation and contraction. Mutations in ATP2A1 cause some autosomal recessive forms of Brody disease(PMID: 23911890, 10914677).

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