Anticorps Recombinant de lapin anti-ATP2B1

ATP2B1 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240848D10

N° de cat : 83827-3-PBS

Synonymes

240848D10, PMCA1, PMCA1kb

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Galerie de données de validation

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  WB analysis of mouse brain using 83827-3-RR (same clone as 83827-3-PBS)

  unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 83827-3-RR (ATP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83827-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 83827-3-RR (same clone as 83827-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83827-3-RR (ATP2B1 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83827-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83827-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83827-3-PBS against Human ATP2B1 was performed. The affinity constant is 4.46 nM.

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  Affinity and Kinetic Characterization of 83827-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83827-3-RR against Human ATP2B1 were performed. The affinity constant is 10.3 nM.

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Informations sur le produit

83827-3-PBS cible ATP2B1 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATP2B1 Protéine recombinante Ag32423
• Nom complet: ATPase, Ca++ transporting, plasma membrane 1
• Masse moléculaire calculée: 135 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: NM_001001323.1
• Symbole du gène: ATP2B1
• Identification du gène (NCBI): 490
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATPase plasma membrane Ca2+ transporting 1 (ATP2B1, also known as plasma membrane Ca2+ pump isoform 1; PMCA1) belongs to the family of ATP-driven calmodulin-dependent Ca2+ pumps that participate in the regulation of intracellular free Ca2+ (PMID:35358416). The ATP2B1 contents of extracellular vesicles are increased in prostate cancer treated with enzalutamide and are negatively regulated by androgen receptors (PMID:29105980). ATP2B1 plays an important role in the prognosis of cholangiocarcinoma. Furthermore, ATP2B1 plays an important role in the prognosis of cholangiocarcinoma and can be a prognostic factor for cholangiocarcinoma (PMID:35875160).

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