Anticorps Polyclonal de lapin anti-ATP2C1

ATP2C1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 13310-1-AP

Synonymes

ATP dependent Ca(2+) pump PMR1, ATP2C1, ATP2C1A, ATPase 2C1, HHD, hSPCA1, KIAA1347, PMR1, PMR1L, SPCA1

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Galerie de données de validation

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  WB analysis using 13310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13310-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 13310-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13310-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 13310-1-AP

  IP Result of anti-ATP2C1 (IP:13310-1-AP, 4ug; Detection:13310-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of mouse brain using 13310-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13310-1-AP (ATP2C1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13310-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13310-1-AP (ATP2C1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13310-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13310-1-AP (ATP2C1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

13310-1-AP cible ATP2C1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP2C1 Protéine recombinante Ag4143
• Nom complet: ATPase, Ca++ transporting, type 2C, member 1
• Masse moléculaire calculée: 919 aa, 101 kDa
• Poids moléculaire observé: 95-103 kDa
• Numéro d’acquisition GenBank: BC028139
• Symbole du gène: ATP2C1
• Identification du gène (NCBI): 27032
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP2C1, also known as hSPCA1, belongs to the family of P-type cation transport ATPases. ATP2C1 is a Golgi-localized ATPase that mediates Golgi uptake of cytosolic Ca(2+) and Mg(2+) and has a role in regulating Ca(2+) and Mn(2+) cellular content (PMID: 11741891). ATP2C1 is found in most tissues except colon, thymus, spleen, and leukocytes. It is expressed in keratinocytes (PMID: 15831496, 14632183). Defects in ATP2C1 cause Hailey-Hailey disease (HHD)(PMID: 10615129, 28035777).

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; A Negative Feedback Loop in Ultraviolet A-Induced Senescence in Human Dermal Fibroblasts Formed by SPCA1 and MAPK.; Authors - Hongfu Xie
human	WB	Clin Cosmet Investig Dermatol; The Pathogenic Mechanism of the ATP2C1 p.Ala109_Gln120del Mutation in Hailey-Hailey Disease; Authors - Peiyao Li
mouse	WB	BMC Pulm Med; Tetramethylpyrazine ameliorates endotoxin-induced acute lung injury by relieving Golgi stress via the Nrf2/HO-1 signaling pathway; Authors - Shaona Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (09-12-2019)

This antibody could detect endogenous ATP2C1 both for WB and IF. For WB, besides the band at the expected molecular weight, it also gives a non specific band at ~ 150 kd.

• Applications: Western Blot, Immunofluorescence,