Anticorps Polyclonal de lapin anti-ATP5D

ATP5D Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 14893-1-AP

Synonymes

ATP5D, F ATPase delta subunit

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Galerie de données de validation

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  WB analysis using 14893-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14893-1-AP (ATP5D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14893-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14893-1-AP (ATP5D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14893-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14893-1-AP (ATP5D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14893-1-AP (ATP5D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 14893-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14893-1-AP (ATP5D Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du poumon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

14893-1-AP cible ATP5D dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP5D Protéine recombinante Ag6682
• Nom complet: ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: BC002389
• Symbole du gène: ATP5D
• Identification du gène (NCBI): 513
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mitochondrial F1Fo-ATP synthase complex uses energy derived from a proton gradient to synthesize ATP. The ATP5D gene encodes the delta subunit of the mitochondrial ATP synthase F1 complex and belongs to the ATPase epsilon chain family. This gene encode the full length protein of 17 kDa with a transit peptide of 22 amino acid.

Publications

Species	Application	Title
mouse	WB	Theranostics; AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages.; Authors - Liangjun Xia
human	WB	Int J Mol Sci; CA9 Silencing Promotes Mitochondrial Biogenesis, Increases Putrescine Toxicity and Decreases Cell Motility to Suppress ccRCC Progression.; Authors - Jiatong Xu
mouse	WB	Front Aging Neurosci; Hippocampus-Based Mitochondrial Respiratory Function Decline Is Responsible for Perioperative Neurocognitive Disorders.; Authors - Keqiang He
human	WB	Am J Physiol Cell Physiol; 3-MST/H2S protects cerebral endothelial cells against OGD/R-induced injury via mitoprotection and inhibition of the RhoA/ROCK pathway.; Authors - Fang Zhang
human	IF	J Neuropathol Exp Neurol; Altered Mitochondria, Protein Synthesis Machinery, and Purine Metabolism Are Molecular Contributors to the Pathogenesis of Creutzfeldt-Jakob Disease.; Authors - Belén Ansoleaga
human	WB	Cell Death Dis; Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.; Authors - Anaïs Aulas