Anticorps Polyclonal de lapin anti-ATP5F1

ATP5F1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15999-1-PBS

Synonymes

ATP5PB, ATP5F 1, ATP synthase subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase peripheral stalk-membrane subunit b

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Galerie de données de validation

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  WB analysis using 15999-1-AP (same clone as 15999-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15999-1-AP (ATP5F1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 15999-1-AP (same clone as 15999-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15999-1-AP (ATP5F1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15999-1-AP (ATP5F1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15999-1-AP (ATP5F1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 15999-1-AP (same clone as 15999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 15999-1-AP (ATP5F1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15999-1-AP (same clone as 15999-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ATP5F1 antibody (15999-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 15999-1-AP (same clone as 15999-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATP5F1 (15999-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 15999-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15999-1-PBS cible ATP5F1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP5F1 Protéine recombinante Ag8571
• Nom complet: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
• Masse moléculaire calculée: 256 aa, 29 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC005366
• Symbole du gène: ATP5F1
• Identification du gène (NCBI): 515
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.

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