Anticorps Recombinant de lapin anti-ATP5H

ATP5H Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250751G4

N° de cat : 86174-2-PBS

Synonymes

ATP synthase peripheral stalk subunit d, ATP5, ATP5JD, ATP5PD, ATPQ

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Galerie de données de validation

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  WB analysis using 86174-2-RR (same clone as 86174-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86174-2-RR (ATP5H antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86174-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86174-2-RR (same clone as 86174-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5H antibody (86174-2-RR, Clone: 250751G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86174-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86174-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86174-2-RR against Human ATP5H were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86174-2-PBS cible ATP5H dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATP5H Protéine recombinante Ag11429
• Nom complet: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
• Masse moléculaire calculée: 137 aa, 16 kDa
• Poids moléculaire observé: 19-22 kDa
• Numéro d’acquisition GenBank: BC032245
• Symbole du gène: ATP5H
• Identification du gène (NCBI): 10476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.

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