Anticorps Recombinant de lapin anti-ATP5I

ATP5I Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250815C6

N° de cat : 86218-2-PBS

Synonymes

ATP synthase membrane subunit e, ATP synthase subunit e, mitochondrial, N-terminally processed, ATP5K, ATP5ME, ATPase subunit e

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Galerie de données de validation

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  WB analysis using 86218-2-RR (same clone as 86218-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86218-2-RR (ATP5I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86218-2-PBS in a different storage buffer formulation.

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  Sample test of MP02299-1

  The mean ATP5I concentration was determined to be 495.7 ng/mL in Hela cell extract based on a 0.9 mg/mL extract load.

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  IF Staining of HeLa using 86218-2-RR (same clone as 86218-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP5I antibody (86218-2-RR, Clone: 250815C6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86218-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02299-1

  Cytometric bead array standard curve of MP02299-1, ATP5I Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86218-2-PBS. Detection antibody: 86218-1-PBS. Standard: Ag9605. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86218-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86218-2-RR against Human ATP5I were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86218-2-PBS cible ATP5I dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATP5I Protéine recombinante Ag9605
• Nom complet: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E
• Masse moléculaire calculée: 69 aa, 8 kDa
• Poids moléculaire observé: 8 kDa
• Numéro d’acquisition GenBank: BC003679
• Symbole du gène: ATP5I
• Identification du gène (NCBI): 521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP5I(ATP synthase subunit e) is also named as ATP5K and belongs to the ATPase e subunit family. The ATP5I gene encodes the e subunit of the mitochondrial ATP synthase Fo complex. Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. Antisense ATP5I in a human HCC cell line inhibited cell growth suggesting that ATP5I acts through the MAP kinase pathway(PMID:11939412).

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