Anticorps Polyclonal de lapin anti-ATP5J

ATP5J Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 14114-1-AP

Synonymes

ATP5, ATP5A, ATP5J, ATPase subunit F6, ATPM, CF6, F6

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Galerie de données de validation

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  WB analysis using 14114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14114-1-AP (ATP5J antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14114-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14114-1-AP (ATP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14114-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14114-1-AP (ATP5J antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14114-1-AP

  IP Result of anti-ATP5J (IP:14114-1-AP, 4ug; Detection:14114-1-AP 1:300) with HEK-293 cells lysate 3680ug.

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  IHC staining of human osteosarcoma using 14114-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14114-1-AP (ATP5J antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human osteosarcoma using 14114-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14114-1-AP (ATP5J antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules SKOV-3, tissu cardiaque humain, tissu hépatique de souris, tissus cardiaques de rat, tissus cardiaques de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu d'ostéosarcome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

14114-1-AP cible ATP5J dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP5J Protéine recombinante Ag5263
• Nom complet: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 9 kDa
• Numéro d’acquisition GenBank: BC066310
• Symbole du gène: ATP5J
• Identification du gène (NCBI): 522
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP5J, also known as coupling factor 6 (CF6), is a soluble integral component of mitochondrial ATP synthase. Mitochondrial ATP synthase is a multi-subunit membrane-bound enzyme that catalyzes the synthesis of ATP by utilizing a proton electrochemical gradient. It consists of three domains, namely the extrinsic and intrinsic membrane domains (F1 and F0, respectively) joined by a stalk. CF6 is one of the subunits in the stalk and an essential component for energy transduction. Recently CF6 has also been reported to play a crucial role in the development of INS resistance and hypertension. CF6 is first synthesized as an immature form in the cytosol, then transported to the mitochondria by an import signal peptide and becomes an active form with the signal peptide cleaved. Western blot analysis of CF6 demonstrates a single band around 9 kDa to 12 kDa in various tissues including heart, liver, brain and HUVEC (human umbilical vein endothelial cells).

Publications

Species	Application	Title
rat	WB	Front Endocrinol (Lausanne); Androgen Excess Induced Mitochondrial Abnormality in Ovarian Granulosa Cells in a Rat Model of Polycystic Ovary Syndrome.; Authors - Linyi Song
mouse	WB	Arch Pharm Res; Overexpressing microRNA-203 alleviates myocardial infarction via interacting with long non-coding RNA MIAT and mitochondrial coupling factor 6.; Authors - Fan Wang
rabbit	WB	J Proteomics; Congenital cataracts affect the retinal visual cycle and mitochondrial function: A multi-omics study of GJA8 knockout rabbits; Authors - Weijie Sun