Anticorps Monoclonal anti-ATP5J2

ATP5J2 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2B3C3

N° de cat : 68128-1-Ig

Synonymes

ATP5J2, ATP5JL

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Galerie de données de validation

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  WB analysis using 68128-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68128-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit heart using 68128-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68128-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 68128-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ATP5J2 antibody (68128-1-Ig, Clone: 2B3C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de rat, cellules HeLa, cellules HepG2, cellules LNCaP, tissu cardiaque de lapin, tissu cardiaque de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

68128-1-Ig cible ATP5J2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATP5J2 Protéine recombinante Ag8811
• Nom complet: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2
• Masse moléculaire calculée: 5-11 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC003678
• Symbole du gène: ATP5J2
• Identification du gène (NCBI): 9551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; N-Acetyltransferase 10 represses Uqcr11 and Uqcrb independently of ac4C modification to promote heart regeneration; Authors - Wenya Ma
human	WB,IHC	J Transl Med; Identification of the metabolic protein ATP5MF as a potential therapeutic target of TNBC; Authors - Kaiyan Chen
human	WB	Cell; YTHDF2 promotes ATP synthesis and immune evasion in B cell malignancies; Authors - Zhenhua Chen

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