Anticorps Polyclonal de lapin anti-ATP6AP1

ATP6AP1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 15305-1-AP

Synonymes

ATP6S1, ATP6 S1, ATP6AP 1, Protein XAP-3, V-ATPase Ac45 subunit

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Galerie de données de validation

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  WB analysis of mouse kidney using 15305-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15305-1-AP (ATP6AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15305-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15305-1-AP (ATP6AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15305-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15305-1-AP (ATP6AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15305-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15305-1-AP (ATP6AP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15305-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15305-1-AP (ATP6AP1 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

15305-1-AP cible ATP6AP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP6AP1 Protéine recombinante Ag7859
• Nom complet: ATPase, H+ transporting, lysosomal accessory protein 1
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 46-52 kDa, 61-64 kDa
• Numéro d’acquisition GenBank: BC000724
• Symbole du gène: ATP6AP1
• Identification du gène (NCBI): 537
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IF	Oncotarget; PMID: 28700346; Authors - Enrica Balza
human	WB	Proteomes; Integrative Proteomic and Phosphoproteomic Analyses of Hypoxia-Treated Pulmonary Artery Smooth Muscle Cells.; Authors - Ang Luo
human	IHC	Sci Rep; ATP6AP1 as a potential prognostic biomarker in CRC by comprehensive analysis and verification; Authors - Shijie Zhang
human	IHC	Cancer Cell Int; Lysosomal gene ATP6AP1 promotes doxorubicin resistance via up-regulating autophagic flux in breast cancer; Authors - Yinjiao Fei
rat	WB	Burns; Role of DLAT in cuproptosis and autophagy in hippocampal tissue of PTSD rats with high-voltage electrical burns; Authors - Chenyang Ge

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Celia (Verified Customer) (01-14-2025)

Great product, speedy delivery and easy order system with help from the team!

• Applications: Western Blot, Immunofluorescence

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