Anticorps Recombinant de lapin anti-ATP6V0A4

ATP6V0A4 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

250861E8

N° de cat : 86321-1-RR

Synonymes

A4, ATP6N1B, ATP6N2, RDRTA2, RTA1C

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Galerie de données de validation

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  WB analysis of rat kidney using 86321-1-RR

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86321-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 86321-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:80000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 86321-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:80000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 86321-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 86321-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86321-1-RR (ATP6V0A4 antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86321-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86321-1-RR against Human ATP6V0A4 were performed. The affinity constant is 0.715 nM.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu rénal de rat
• Résultats positifs en IHC: tissu rénal humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:10000-1:40000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86321-1-RR cible ATP6V0A4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATP6V0A4 Protéine recombinante Ag16095
• Nom complet: ATPase, H+ transporting, lysosomal V0 subunit a4
• Masse moléculaire calculée: 840 aa, 96 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC109305
• Symbole du gène: ATP6V0A4
• Identification du gène (NCBI): 50617
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ATP6V0A4 is a component of vacuolar-H+ATPase (V-ATPase) which is a multi-subunit enzyme that couples ATP hydrolysis to proton pumping across membranes. The V-ATPases are comprised of two major parts, the cytosolic V1 domain involved in ATP-binding and subsequent hydrolysis, and the membrane-associated V0 domain responsible for proton translocation. The V0 domain is composed of five subunits: a, c, c', c'' and d. The 'a' subunit of V0 domain has four isoforms : a1-a4. It has been found that mutations in ATP6V0A4(a4) are associated with distal renal tubular acidosis(dRTA) combined in some cases with progressive hearing loss leading to sensorineural deafness.

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