Anticorps Monoclonal anti-ATP6V0D1

ATP6V0D1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G7A12

N° de cat : 68506-1-PBS

Synonymes

32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD

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Galerie de données de validation

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  WB analysis using 68506-1-Ig (same clone as 68506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

  IP result of anti-ATP6V0D1 (IP:68506-1-Ig, 4ug; Detection:68506-1-Ig 1:3000) with HeLa cells lysate 1520 ug. This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68506-1-Ig (same clone as 68506-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68506-1-Ig (same clone as 68506-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V0D1 antibody (68506-1-Ig, Clone: 1G7A12 ) at dilution of 1:2400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68506-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68506-1-PBS cible ATP6V0D1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris

• Réactivité: Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATP6V0D1 Protéine recombinante Ag13012
• Nom complet: ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
• Masse moléculaire calculée: 351 aa, 40 kDa
• Poids moléculaire observé: 37-41 kDa
• Numéro d’acquisition GenBank: BC008861
• Symbole du gène: ATP6V0D1
• Identification du gène (NCBI): 9114
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

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