Anticorps Polyclonal de lapin anti-ATP6V1A

ATP6V1A Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 51152-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 51152-1-AP (same clone as 51152-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 51152-1-AP (same clone as 51152-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 51152-1-AP (same clone as 51152-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 51152-1-AP (same clone as 51152-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 51152-1-AP (same clone as 51152-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51152-1-AP (ATP6V1A Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 51152-1-AP (same clone as 51152-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51152-1-AP (ATP6V1A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 51152-1-PBS in a different storage buffer formulation.

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Informations sur le produit

51152-1-PBS cible ATP6V1A dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: NM_001690
• Symbole du gène: ATP6V1A
• Identification du gène (NCBI): 523
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.This antibody is specific to ATP6V1A.

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