Anticorps Monoclonal anti-ATP6V1A

• Phare
• Validé par KD/KO

ATP6V1A Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C5G2

N° de cat : 68440-1-PBS

Synonymes

V-ATPase 69 kDa subunit, VA68, EC:7.1.2.2, ATP6V1A1, ATP6A1

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Galerie de données de validation

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  WB analysis using 68440-1-Ig (same clone as 68440-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68440-1-Ig (ATP6V1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68440-1-Ig (same clone as 68440-1-PBS)

  WB result of ATP6V1A antibody (68440-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1A transfected HeLa cells. This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68440-1-Ig (same clone as 68440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68440-1-Ig (same clone as 68440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68440-1-Ig (same clone as 68440-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATP6V1A Monoclonal antibody (68440-1-Ig, Clone:1C5G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68440-1-PBS cible ATP6V1A dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATP6V1A Protéine recombinante Ag10820
• Nom complet: ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• Masse moléculaire calculée: 617 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC013138
• Symbole du gène: ATP6V1A
• Identification du gène (NCBI): 523
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.

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