Anticorps Polyclonal de lapin anti-ATP6V1B1

ATP6V1B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 14780-1-AP

Synonymes

V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1

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Galerie de données de validation

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  WB analysis using 14780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14780-1-AP (ATP6V1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14780-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14780-1-AP (ATP6V1B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14780-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14780-1-AP (ATP6V1B1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 14780-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14780-1-AP (ATP6V1B1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293 using 14780-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP6V1B1 antibody (14780-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

14780-1-AP cible ATP6V1B1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP6V1B1 Protéine recombinante Ag6332
• Nom complet: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC063411
• Symbole du gène: ATP6V1B1
• Identification du gène (NCBI): 525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.

Publications

Species	Application	Title
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
mouse	IF	Dis Model Mech; Expression of N471D strumpellin leads to defects in the endolysosomal system.; Authors - Lin Song
human	WB	J Cell Physiol; Differential regulation of vacuolar H+ -ATPase subunits by transforming growth factor-β1 in salivary ducts.; Authors - Milena Kluge
mouse	WB	Environ Int; Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury; Authors - Xing Zhang
human	WB,IHC	Mol Cell Biochem; ATP6V1B1 regulates ovarian cancer progression and cisplatin sensitivity through the mTOR/autophagy pathway; Authors - Shien Mo ; KD Validated
mouse	IF	Physiol Rep; Expression and localization of the mechanosensitive/osmosensitive ion channel TMEM63B in the mouse urinary tract; Authors - Marianela G Dalghi

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