Anticorps Monoclonal anti-ATP6V1B1

ATP6V1B1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G10A8

N° de cat : 68219-1-PBS

Synonymes

ATP6B1, ATP6V1B1, RTA1B, V ATPase subunit B 1, VATB, VMA2, VPP3

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Galerie de données de validation

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  WB analysis using 68219-1-Ig (same clone as 68219-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68219-1-Ig (ATP6V1B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68219-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68219-1-Ig (same clone as 68219-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP6V1B1 antibody (68219-1-Ig, Clone: 1G10A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68219-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68219-1-PBS cible ATP6V1B1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATP6V1B1 Protéine recombinante Ag7405
• Nom complet: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC063411
• Symbole du gène: ATP6V1B1
• Identification du gène (NCBI): 525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.

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