Anticorps Recombinant de lapin anti-ATP6V1B1

ATP6V1B1 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240955B9

N° de cat : 83953-5-PBS

Synonymes

V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1

---

Galerie de données de validation

• 

  WB analysis using 83953-5-RR (same clone as 83953-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 83953-5-RR (same clone as 83953-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 83953-5-RR (same clone as 83953-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 83953-5-RR (same clone as 83953-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 83953-5-RR (same clone as 83953-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

---

Informations sur le produit

83953-5-PBS cible ATP6V1B1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATP6V1B1 Protéine recombinante Ag6332
• Nom complet: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC063411
• Symbole du gène: ATP6V1B1
• Identification du gène (NCBI): 525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.

{{ptg:RelatedPrimaryAntibodies}}