Anticorps Polyclonal de lapin anti-ATP6V1C1

ATP6V1C1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 16054-1-AP

Synonymes

ATP6C, ATP6D, VATC, V-ATPase subunit C 1, Vma5

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 16054-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 16054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16054-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16054-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 16054-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 16054-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below

Informations sur le produit

16054-1-AP cible ATP6V1C1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP6V1C1 Protéine recombinante Ag9020
• Nom complet: ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
• Masse moléculaire calculée: 382 aa, 44 kDa
• Poids moléculaire observé: 42-44 kDa
• Numéro d’acquisition GenBank: BC010960
• Symbole du gène: ATP6V1C1
• Identification du gène (NCBI): 528
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The vacuolar H+-ATPase (V-ATPase) is a functionally conserved multimeric complex localized at the membranes of many organelles where its proton-pumping action is required for proper lumen acidification (PMID: 39210597). ATP6V1C1, also known as ATPase, H+ transporting, lysosomal V1 subunit C1, is a component of the V1 sector of the V-ATPase complex. ATP6v1c1 knockdown significantly reduces tumor stimulated bone resorption through osteoclastogenesis at the bone and metastasis in vivo, as well as V-ATPase activity, proliferation, and mTORC1 activation in vitro (PMID: 28504970). Also, ATP6V1C1, associated with the tumor microenvironment and mTORC1 signaling pathway, is a potential diagnostic, prognostic, and therapeutic biomarker for hepatocellular carcinoma () (PMID: 39557733).

Publications

Species	Application	Title
human	WB	Mol Ther; Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.; Authors - Benfu Zhong
mouse	WB	J Biol Chem; A brain-enriched Drp1 isoform associates with lysosomes, late endosomes and the plasma membrane.; Authors - Kie Itoh
human	WB	Commun Biol; A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets.; Authors - Masashi Watanabe
human	WB	Biochem Biophys Res Commun; Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells.; Authors - Jong-Jer Lee
human	IHC	Bioengineered; A novel four-gene of iron metabolism-related and methylated for prognosis prediction of hepatocellular carcinoma.; Authors - Huimin Shen
human	WB	Autophagy; Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate; Authors - Mengting Ou

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