Anticorps Recombinant de lapin anti-ATP6V1C1

ATP6V1C1 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250958C3

N° de cat : 86345-2-PBS

Synonymes

ATP6C, ATP6D, VATC, V-ATPase subunit C 1, Vma5

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Galerie de données de validation

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  WB analysis using 86345-2-RR (same clone as 86345-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86345-2-PBS in a different storage buffer formulation.

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  Sample test of MP02350-1

  The mean ATP6V1C1 concentration was determined to be 29.6 ng/mL in NIH/3T3 cell extract based on a 1.4 mg/mL extract load.

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  IHC staining of mouse brain using 86345-2-RR (same clone as 86345-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86345-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 86345-2-RR (same clone as 86345-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86345-2-RR (ATP6V1C1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86345-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02350-1

  Cytometric bead array standard curve of MP02350-1, ATP6V1C1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86345-2-PBS. Detection antibody: 86345-1-PBS. Standard: Ag9020. Range: 1.562-200 ng/mL.

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  Affinity and Kinetic Characterization of 86345-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86345-2-RR against Human ATP6V1C1 were performed. The affinity constant is 8.06 nM.

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Informations sur le produit

86345-2-PBS cible ATP6V1C1 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATP6V1C1 Protéine recombinante Ag9020
• Nom complet: ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
• Masse moléculaire calculée: 382 aa, 44 kDa
• Poids moléculaire observé: 42-44 kDa
• Numéro d’acquisition GenBank: BC010960
• Symbole du gène: ATP6V1C1
• Identification du gène (NCBI): 528
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The vacuolar H+-ATPase (V-ATPase) is a functionally conserved multimeric complex localized at the membranes of many organelles where its proton-pumping action is required for proper lumen acidification (PMID: 39210597). ATP6V1C1, also known as ATPase, H+ transporting, lysosomal V1 subunit C1, is a component of the V1 sector of the V-ATPase complex. ATP6v1c1 knockdown significantly reduces tumor stimulated bone resorption through osteoclastogenesis at the bone and metastasis in vivo, as well as V-ATPase activity, proliferation, and mTORC1 activation in vitro (PMID: 28504970). Also, ATP6V1C1, associated with the tumor microenvironment and mTORC1 signaling pathway, is a potential diagnostic, prognostic, and therapeutic biomarker for hepatocellular carcinoma () (PMID: 39557733).

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