Anticorps Polyclonal de lapin anti-ATP6V1D

ATP6V1D Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 14920-1-AP

Synonymes

ATP6M, ATP6V1D, V ATPase subunit D, V type proton ATPase subunit D, Vacuolar proton pump subunit D, VATD, VMA8

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Galerie de données de validation

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  WB analysis of human brain using 14920-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 14920-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14920-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14920-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14920-1-AP (ATP6V1D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 14920-1-AP

  IP Result of anti-ATP6V1D (IP:14920-1-AP, 4ug; Detection:14920-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human lung cancer using 14920-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14920-1-AP (ATP6V1D antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 14920-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14920-1-AP (ATP6V1D antibody) at dilution of 1:100 (under 10x lens).

• 

  FC experiment of HeLa using 14920-1-AP

  1X10^6 HeLa cells were stained with 0.2ug ATP6V1D antibody (14920-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu de muscle squelettique de souris, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

14920-1-AP cible ATP6V1D dans les applications de WB, IP, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP6V1D Protéine recombinante Ag6737
• Nom complet: ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC001411
• Symbole du gène: ATP6V1D
• Identification du gène (NCBI): 51382
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP6V1D is also named as ATP6M, VATD(V-type proton ATPase subunit D) and belongs to the V-ATPase D subunit family. ATP6V1D gene has been under strong negative selection during evolution and is highly conserved among mammals, flies, worms, yeast, plants, and bacteria(PMID:11435709). It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

Publications

Species	Application	Title
mouse	WB	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio
rat	WB	J Proteome Res; Quantitative proteome of medulla oblongata in spontaneously hypertensive rats.; Authors - Sun Dongmei D
human	WB	Mol Cell Proteomics; Multi Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal Proteins; Authors - Fatema Akter
human	WB	Autophagy; Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate; Authors - Mengting Ou
human	WB	Nat Commun; Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo; Authors - Jasjot Singh