Anticorps Polyclonal de lapin anti-ATP6V1E1

• Phare
• Validé par KD/KO

ATP6V1E1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 15280-1-AP

Synonymes

ATP6E, ATP6E2, ATP6V1E, ATP6V1E1, P31, V ATPase 31 kDa subunit, V ATPase subunit E 1, Vma4

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Galerie de données de validation

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  WB analysis of HeLa using 15280-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15280-1-AP (ATP6V1E1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15280-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15280-1-AP (ATP6V1E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15280-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15280-1-AP (ATP6V1E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15280-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15280-1-AP (ATP6V1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15280-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15280-1-AP (ATP6V1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15280-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15280-1-AP (ATP6V1E1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15280-1-AP

  IP Result of anti-ATP6V1E1 (IP:15280-1-AP, 3ug; Detection:15280-1-AP 1:500) with mouse brain tissue lysate 6000ug.

• 

  IHC staining of mouse testis using 15280-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15280-1-AP (ATP6V1E1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15280-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15280-1-AP (ATP6V1E1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 15280-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15280-1-AP (ATP6V1E1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15280-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15280-1-AP (ATP6V1E1 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, tissu cérébral de souris, tissu cérébral humain, tissu hépatique humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15280-1-AP cible ATP6V1E1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATP6V1E1 Protéine recombinante Ag7399
• Nom complet: ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC004443
• Symbole du gène: ATP6V1E1
• Identification du gène (NCBI): 529
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP6V1E1, also named as ATP6E, ATP6E2 and p31, belongs to the V-ATPase E subunit family. It is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase and the lack of mycobacterial phagosome acidification are directly attributed to the Mtb protein tyrosine phosphatase PtpA.

Publications

Species	Application	Title
human	WB,IF	Proc Natl Acad Sci U S A; Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.; Authors - Wong Dennis D ; KO Validated
human	WB	J Cell Sci; Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.; Authors - Dolma Choezom
pig	WB	Microorganisms; Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.; Authors - Lanxin Yuan
human	WB, IHC	J Proteomics; iTRAQ-based quantitative proteomic analysis for identification of oligodendroglioma biomarkers related with loss of heterozygosity on chromosomal arm 1p.; Authors - Yang Liu-song LS
human	WB	Vet Res; Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.; Authors - Bingxin Chu
human	WB	Nat Commun; Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly; Authors - Edoardo Ratto

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sven (Verified Customer) (04-14-2022)

Works very well on western blot for mouse tissue (1:1000 dilution in TBS-T with 5% BSA, 0.02% NaN3).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 dilution
• Cell Tissue Type: Mouse Embryonic Fibroblasts (MEFs)

ATP6V1E1 Antibody Western Blot validation (1:1000 dilution dilution) in Mouse Embryonic Fibroblasts (MEFs) (Cat no:15280-1-AP)