Anticorps Recombinant de lapin anti-ATP6V1E1

ATP6V1E1 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240584G12

N° de cat : 83636-6-PBS

Synonymes

V-ATPase subunit E 1, V-ATPase 31 kDa subunit, P31, ATP6E2, ATP6E

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Galerie de données de validation

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  WB analysis using 83636-6-RR (same clone as 83636-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83636-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83636-6-RR (same clone as 83636-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83636-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83636-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83636-6-RR against Human ATP6V1E1 were performed. The affinity constant is 5.43 nM.

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Informations sur le produit

83636-6-PBS cible ATP6V1E1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ATP6V1E1 Protéine recombinante Ag7399
• Nom complet: ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC004443
• Symbole du gène: ATP6V1E1
• Identification du gène (NCBI): 529
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP6V1E1, also named as ATP6E, ATP6E2 and p31, belongs to the V-ATPase E subunit family. It is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase and the lack of mycobacterial phagosome acidification are directly attributed to the Mtb protein tyrosine phosphatase PtpA.

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