Anticorps Monoclonal anti-ATP6V1H

ATP6V1H Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3G7B5

N° de cat : 68425-1-PBS

Synonymes

ATP6V1H, CGI 11, MSTP042, NBP1, Nef binding protein 1, SFD, SFDalpha, SFDbeta, V ATPase 50/57 kDa subunits, V ATPase subunit H, V type proton ATPase subunit H, Vacuolar proton pump subunit H, VMA13

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Galerie de données de validation

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  WB analysis using 68425-1-Ig (same clone as 68425-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68425-1-Ig (ATP6V1H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68425-1-PBS in a different storage buffer formulation.

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  WB analysis using 68425-1-Ig (same clone as 68425-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68425-1-Ig (ATP6V1H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68425-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68425-1-Ig (same clone as 68425-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATP6V1H antibody (68425-1-Ig, Clone: 3G7B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68425-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68425-1-PBS cible ATP6V1H dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATP6V1H Protéine recombinante Ag10130
• Nom complet: ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
• Masse moléculaire calculée: 483 aa, 56 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC025275
• Symbole du gène: ATP6V1H
• Identification du gène (NCBI): 51606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains.

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