Anticorps Polyclonal de lapin anti-ATP7A-Specific

ATP7A-Specific Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55375-1-PBS

Synonymes

ATP7A, ATPase, Cu++ transporting, alpha polypeptide, Copper transporting ATPase 1

---

Galerie de données de validation

• 

  WB analysis using 55375-1-AP (same clone as 55375-1-PBS)

  Unboiled mouse lung tissue were subjected to SDS PAGE followed by western blot with 55375-1-AP (ATP7A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55375-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse lung using 55375-1-AP (same clone as 55375-1-PBS)

  IP result of anti-ATP7A-Specific (IP:55375-1-AP, 4ug; Detection:55375-1-AP 1:500) with mouse lung tissue lysate 1520 ug. This data was developed using the same antibody clone with 55375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 55375-1-AP (same clone as 55375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55375-1-AP (ATP7A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55375-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

55375-1-PBS cible ATP7A-Specific dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ATPase, Cu++ transporting, alpha polypeptide
• Masse moléculaire calculée: 165 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: NM_000052
• Symbole du gène: ATP7A
• Identification du gène (NCBI): 538
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATPase copper transporting alpha(ATP7A) is a type of transmembrane P-type ATPase for copper transport. ATP7A and ATP7B are homologous genes responsible for copper transport across cell membranes. ATP7A transports copper ions from the Golgi membrane to copper-dependent enzymes (such as dopamine β-hydroxylase) in the secretory pathway. When copper ion concentrations rise, ATP7A relocalizes from the Golgi apparatus to the plasma membrane, promoting copper efflux and thereby protecting cells from copper toxicity. Mutations in ATP7A are associated with Menkes disease (MNK)( PMID: 28389643, 30341172).

{{ptg:RelatedPrimaryAntibodies}}