Anticorps Recombinant de lapin anti-ATP7B-Specific

ATP7B-Specific Recombinant Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242827B3

N° de cat : 85288-2-PBS

Synonymes

ATP7B, 242827B3, ATPase, Cu++ transporting, beta polypeptide, Copper-transporting ATPase 2, EC:7.2.2.8

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Galerie de données de validation

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  WB analysis using 85288-2-RR (same clone as 85288-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85288-2-RR (ATP7B-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85288-2-RR (same clone as 85288-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85288-2-RR (ATP7B-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85288-2-RR (same clone as 85288-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85288-2-RR (ATP7B-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 85288-2-RR (same clone as 85288-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ATP7B-Specific antibody (85288-2-RR, Clone: 242827B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 85288-2-RR (same clone as 85288-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ATP7B-Specific Recombinant antibody (85288-2-RR, Clone:242827B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85288-2-PBS in a different storage buffer formulation.

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Informations sur le produit

85288-2-PBS cible ATP7B-Specific dans les applications de WB, IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ATPase, Cu++ transporting, beta polypeptide
• Masse moléculaire calculée: 157 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: NM_000053
• Symbole du gène: ATP7B
• Identification du gène (NCBI): 540
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP7B, also named as PWD, WC1 and WND, belongs to the cation transport ATPase (P-type) family and Type IB subfamily. ATP7B is involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. ATP7B catalyzes the reaction: ATP + H2O + Cu2+(In) = ADP + phosphate + Cu2+(Out). Defects in ATP7B are the cause of Wilson disease (WD). This antibody is specific to ATP7B.

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