Anticorps Monoclonal anti-ATPAF2

ATPAF2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H9A9

N° de cat : 68351-1-PBS

Synonymes

ATP12, LP3663, ATP12p, ATP12 homolog, 1H9A9

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Galerie de données de validation

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  WB analysis using 68351-1-Ig (same clone as 68351-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68351-1-Ig (ATPAF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68351-1-Ig (same clone as 68351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68351-1-Ig (ATPAF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68351-1-Ig (same clone as 68351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68351-1-Ig (ATPAF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68351-1-Ig (same clone as 68351-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATPAF2 antibody (68351-1-Ig, Clone: 1H9A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68351-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50625-1

  Cytometric bead array standard curve of MP50625-1, ATPAF2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68351-1-PBS. Detection antibody: 68351-2-PBS. Standard:Ag29065. Range: 0.098-6.25 ng/mL.

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Informations sur le produit

68351-1-PBS cible ATPAF2 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATPAF2 Protéine recombinante Ag29065
• Nom complet: ATP synthase mitochondrial F1 complex assembly factor 2
• Masse moléculaire calculée: 289 aa, 33 kDa
• Poids moléculaire observé: ~30 kDa
• Numéro d’acquisition GenBank: BC032126
• Symbole du gène: ATPAF2
• Identification du gène (NCBI): 91647
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) also name as ATP12 and ATP12p, is an essential house keeping protein. ATPAF2 is an assembly factor for the F1 component of mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. The calculated MW is 33 kDa, 68351-1-Ig can detect bands around 30 kDa and 50 kDa, the larger band may correspond to dimer or complex form. (PMID: 1826907, 11410595)

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