Anticorps Monoclonal anti-ATPB

ATPB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A11B9

N° de cat : 66600-1-PBS

Synonymes

1A11B9, ATP synthase F1 subunit beta, ATP5B, ATP5F1B, ATPMB

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Galerie de données de validation

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  WB analysis using 66600-1-Ig (same clone as 66600-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66600-1-Ig (ATPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66600-1-Ig (same clone as 66600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66600-1-Ig (same clone as 66600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 66600-1-Ig (same clone as 66600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 66600-1-Ig (same clone as 66600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 66600-1-Ig (same clone as 66600-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66600-1-Ig (same clone as 66600-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66600-1-Ig (same clone as 66600-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66600-1-PBS cible ATPB dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATPB Protéine recombinante Ag11177
• Nom complet: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
• Masse moléculaire calculée: 529 aa, 57 kDa
• Poids moléculaire observé: 45-57 kDa
• Numéro d’acquisition GenBank: BC016512
• Symbole du gène: ATPB
• Identification du gène (NCBI): 506
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.

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