Anticorps Polyclonal de lapin anti-ATPBD4

ATPBD4 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 23993-1-AP

Synonymes

ATP binding domain 4, ATPBD4, Diphthamide synthase, Diphthamide synthetase, Diphthine-ammonia ligase, DPH6, Protein DPH6 homolog

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 23993-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23993-1-AP (ATPBD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 23993-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 23993-1-AP (ATPBD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 23993-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 23993-1-AP (ATPBD4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules L02
• Résultats positifs en IF/ICC: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

23993-1-AP cible ATPBD4 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATPBD4 Protéine recombinante Ag21178
• Nom complet: ATP binding domain 4
• Masse moléculaire calculée: 267 aa, 30 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC008485
• Symbole du gène: ATPBD4
• Identification du gène (NCBI): 89978
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Diphthamide is a highly modified histidine in the transcription factor EEF2 that is conserved from yeast to human. ATPBD4, also known as DPH6, belongs to the Diphthine--ammonia ligase family. DPH6 amidase may catalyze the last step of diphthamide biosynthesis using ammonium and ATP to play a role in catalyzing the final step of diphthamide biosynthesis, amidation of diphthine to diphthamide (PubMed:23169644).

Publications

Species	Application	Title
human	WB	Brain Sci; Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells.; Authors - Tsung-Ming Hu

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