Anticorps Monoclonal anti-ATPBD4

ATPBD4 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B12E1

N° de cat : 68397-1-PBS

Synonymes

ATP binding domain 4, ATPBD4, DPH6

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Galerie de données de validation

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  WB analysis using 68397-1-Ig (same clone as 68397-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68397-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 68397-1-Ig (same clone as 68397-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68397-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68397-1-PBS cible ATPBD4 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATPBD4 Protéine recombinante Ag33218
• Nom complet: ATP binding domain 4
• Masse moléculaire calculée: 267 aa, 30 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC008485
• Symbole du gène: ATPBD4
• Identification du gène (NCBI): 89978
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Diphthamide is a highly modified histidine in the transcription factor EEF2 that is conserved from yeast to human. ATPBD4, also known as DPH6, belongs to the Diphthine--ammonia ligase family. DPH6 amidase may catalyze the last step of diphthamide biosynthesis using ammonium and ATP to play a role in catalyzing the final step of diphthamide biosynthesis, amidation of diphthine to diphthamide (PubMed:23169644).

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