Anticorps Polyclonal de lapin anti-ATR

• Phare
• Validé par KD/KO

ATR Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19787-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 19787-1-AP (same clone as 19787-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19787-1-AP (ATR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19787-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 19787-1-AP (same clone as 19787-1-PBS)

  WB result of ATR antibody (19787-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATR transfected HeLa cells. This data was developed using the same antibody clone with 19787-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 19787-1-AP (same clone as 19787-1-PBS)

  IP result of anti-ATR (IP:19787-1-AP, 4ug; Detection:19787-1-AP 1:500) with mouse testis tissue lysate 2040 ug. This data was developed using the same antibody clone with 19787-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19787-1-PBS cible ATR dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ataxia telangiectasia and Rad3 related
• Masse moléculaire calculée: 301 kDa
• Poids moléculaire observé: 250-290 kDa
• Numéro d’acquisition GenBank: NM_001184
• Symbole du gène: ATR
• Identification du gène (NCBI): 545
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ATR, also named as FRP1, belongs to the PI3/PI4-kinase family and ATM subfamily. ATR is a serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR recognizes the substrate consensus sequence [ST]-Q. ATR phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. It is required for FANCD2 ubiquitination. It is critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. ATR catalyze the reaction: ATP + a protein = ADP + a phosphoprotein. Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) which is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. The antibody can recognize all the isoforms of ATR.

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