Anticorps Polyclonal de lapin anti-ATXN10

ATXN10 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF,ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15693-1-AP

Synonymes

ataxin 10, ATXN10, Brain protein E46 homolog, E46L, SCA10

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Galerie de données de validation

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  WB analysis of human brain using 15693-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 15693-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15693-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 15693-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15693-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 15693-1-AP

  IP Result of anti-ATXN10 (IP:15693-1-AP, 4ug; Detection:15693-1-AP 1:1000) with A431 cells lysate 1200ug.

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  IHC staining of human ovary tumor using 15693-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 15693-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 15693-1-AP

  Immunofluorescent analysis of Hela cells, using ATXN10 antibody 15693-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HEK-293, cellules HeLa, tissu de cervelet humain, tissu rénal humain
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

15693-1-AP cible ATXN10 dans les applications de WB, IP, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATXN10 Protéine recombinante Ag8259
• Nom complet: ataxin 10
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC007508
• Symbole du gène: ATXN10
• Identification du gène (NCBI): 25814
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.

Publications

Species	Application	Title
mouse	WB	Cell; Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways.; Authors - Sang Liyun L
human	WB	J Neurochem; Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms.; Authors - Robert J Andrew