Anticorps Polyclonal de lapin anti-AUP1

AUP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 13726-1-AP

Synonymes

ancient ubiquitous protein 1, Lipid droplet-regulating VLDL assembly factor AUP1

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Galerie de données de validation

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  WB analysis of K-562 using 13726-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13726-1-AP (AUP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13726-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13726-1-AP (AUP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13726-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13726-1-AP (AUP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13726-1-AP

  IP result of anti-AUP1 (IP:13726-1-AP, 3ug; Detection:13726-1-AP 1:500) with HEK-293 cells lysate 880ug.

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  IHC staining of mouse brain using 13726-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13726-1-AP (AUP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 13726-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AUP1 antibody (13726-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, cellules Jurkat
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

13726-1-AP cible AUP1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AUP1 Protéine recombinante Ag4680
• Nom complet: ancient ubiquitous protein 1
• Masse moléculaire calculée: 416 aa, 45 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC033646
• Symbole du gène: AUP1
• Identification du gène (NCBI): 550
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AUP1 (Ancient ubiquitous protein 1) is a protein associated with lipid metabolism, which is widely expressed on the surface of ER and Lipid droplets, involved in the degradation of misfolded proteins through autophagy (PMID: 37029364). In addition, AUP1 also controls lipid synthesis as it induces ubiquitination and the subsequent degradation of several key regulators of lipid biosynthesis, such as the cholesterol biosynthetic enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (PMID: 28330944).

Publications

Species	Application	Title
human	WB	Mol Biol Cell; Lipid disequilibrium disrupts ER proteostasis by impairing ERAD substrate glycan trimming and dislocation.; Authors - Milton To
human	WB	PLoS One; Chlamydia trachomatis Infection Leads to Defined Alterations to the Lipid Droplet Proteome in Epithelial Cells.; Authors - Hector Alex Saka
human	WB	Mol Biol Cell; A VCP inhibitor substrate trapping approach (VISTA) enables proteomic profiling of endogenous ERAD substrates.; Authors - Edmond Y Huang
human	WB	bioRxiv; Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations; Authors - Sihang Zhou
human	WB	mBio; AUP1 and UBE2G2 complex targets STING signaling and regulates virus-induced innate immunity; Authors - Xin-Tao Wang
human		Mol Cell; Genome-wide CRISPR Analysis Identifies Substrate-Specific Conjugation Modules in ER-Associated Degradation.; Authors - Dara E Leto

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